http://imagej.273.s1.nabble.com/Focus-measurement-more-general-image-analysis-question-tp3700123p3700125.html
definition. It's for sure that our microscope needs to undergo
> You are tackling an interesting question. To be in focus is always meant
> for an object, adjust focus is meant for a whole image. Hence the
> problem with focus is the observer, a cell of certain thickness might be
> in focus at the cell border or at the cell center.
>
> However, still there is a more important question. Images from confocal
> microscopes are by definition in focus of course related to the
> 'pin-hole' size. If you call such images in 'bad focus' maybe there is
> some help in microscope adjustment necessary?
> If you have stacks of images, images taken at different focal
> adjustments there is a program to merge such stacks into one image (e.g.
> plugin Exteded depth of field). However, my first remark is still valid,
> meaning the outcome of such a method is possibly far away from expectation.
> Regards
> Karsten
>
> Am 06.03.2007 um 09:17 schrieb Antje:
>
>> Hello,
>>
>> sorry, the question is not that strongly related with ImageJ but maybe
>> there is still someone who can help?
>>
>> I have a huge set of microscopic images from a confocal microscope
>> (cells/nuclei + marker) and I'd like to automatically filter images
>> which have a bad focus. First, I was thinking about some gradient
>> values to judge but they are so dependent of the content (number of
>> cells... , presence of the marker). Is there any reliable method known
>> to measure and compare the focus of images??? Or does anybody know
>> papers dealing with this issue???
>> (If there is something available in ImageJ it would be fine but I
>> could not find anything...)
>>
>> Antje
>>
>>
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