Re: How to do frame averaging?

Posted by Tony Collins-4 on
URL: http://imagej.273.s1.nabble.com/How-to-do-frame-averaging-tp3700186p3700188.html

I agree. I assumed the reference to live cell imaging means the images
are part of a video sequence. But your right - if this is just 30 images
of the same frame, a simple average would be best.

Cheers,

Tony

Tony J. Collins, Ph.D.
McMaster Biophotonics Facility
Dept. Biochemistry and Biomedical Sciences HSC 4H21A
McMaster University, Hamilton, ON, L8N 3Z5
[phone] x28812(off.)/x26488(lab)
[hidden email]     www.macbiophotonics.ca

> -----Original Message-----
> From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
> Gabriel Landini
> Sent: Sunday, March 04, 2007 3:08 PM
> To: [hidden email]
> Subject: Re: How to do frame averaging?
>
> On Sunday 04 March 2007 17:12:45 Tony Collins wrote:
> > There's Nico Stuurman's Running Zprojector plugin:
> > http://valelab.ucsf.edu/~nico/IJplugins/Running_ZProjector.html
> > Have a look at Christopher Philip Mauer's Kalman Filter plugin too.
> > http://rsb.info.nih.gov/ij/plugins/kalman.html
>
> I think that 'running averages' (what the running Z projector does)

and
> Kalman
> filter are to be used for video *sequences* or streams, not for static
> series
> of images of the same field.
> Those two techniques give different weights to each frame in the
stream,
> but
> if one wants is to reduce the random element between shots that do not
> change
> to increase S/N, just averaging all the fields is more appropriate.
>
> Cheers,
>
> G.