Re: How to do frame averaging?

Posted by Gabriel Landini on
URL: http://imagej.273.s1.nabble.com/How-to-do-frame-averaging-tp3700186p3700192.html

On Sunday 04 March 2007, Mikinka wrote:
> I am doing live cell imaging and I would like to do frame averaging such as
> getting average of 30 image frames to improve signal to background noise
> ratios. I'm new to ImageJ. Any help would be greatly appreciated.

Ideally you should this at the camera (some cameras have an integrator) or
done with the software provided with the manufacturer.

If these are non available, then the alternative is:

grab n images,
convert the images to a stack,
do a stack Z projection using "Average".

This will reduce random image noise by a factor of sqrt(n) but may add some
small degree of blurring.

Cheers,
G.