Re: "Grayscale" values for RGB images

Posted by karo03 on Feb 23, 2007; 9:29pm
URL: http://imagej.273.s1.nabble.com/Grayscale-values-for-RGB-images-tp3700260p3700262.html

Possibly it might not help for further clarification but:
1. IMHO for real (aka exact) fluorescence measurement a b/w camera is  
best with adjustable parameters since for measurement the integral  
intensity is the object of interest, which are ruled by the filters  
of the microscope! Using a color camera the fluorescence 'spectrum'  
is divided by the rgb filters inbuilt in the camera (and additionally  
corrupted by the pixel arrangement system of the camera).
2. Having a color camera to have impressive colors contradicts exact  
measurement. Hence better microscopes are equipped by a color camera  
and a measure camera or only a measure camera with a quick rgb scan  
inbuilt for visualization.
3. Handling data unluckily gathered with a rgb ccd camera I recommend  
to use the channel (weight 1!) with the most prominent signal (e.g.  
FITS green). Not to loose information I am measuring the red and the  
blue channel additionally separately (so to say each with weight 1)!  
This can serve for quality control (microscope/filter adjustment,  
labelling) or possibly there is in the red part of FITS a Noble price  
hidden?!?

best regards
Karsten

Am 19.02.2007 um 06:12 schrieb Jacqui Ross:

> Or should I be using RGB weighting? If so, how should this be  
> determined
> experimentally for the camera?
>
> If someone can clarify when weighting should be applied, that would be
> helpful.