Re: "Grayscale" values for RGB images

Posted by Dimiter Prodanov on Feb 20, 2007; 9:28am
URL: http://imagej.273.s1.nabble.com/Grayscale-values-for-RGB-images-tp3700260p3700267.html

Hi Jacqui,

If you have single channel, like FITC my advise is to use
only the intensity of of the green pixels; so you arrive
at 1*G+0*R+0*B.
If you have data on 2 channels: like one primary and some "bleeding"
to a second one use 0.5*G+0.5*R+0*B.
But here you have to
bear ain mind the different sensitivity of the camera in the different
parts of the the spectrum. So if you expected that your camera is
less sensitive in the red you should increase the weight of the R >0.5.
Cheers

Dimiter


_______________________________________________________________________
Dr Dimiter Prodanov, MD, Ph.D.

Neural Engineering Rehabilitation Laboratory
(Laboratoire de Génie de la Réhabilitation Neurale)
Département de Physiologie et Pharmacologie
Université catholique de Louvain
Avenue Hippocrate, 54
POBox UCL-5446 / B-1200 Bruxelles -Belgique-
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