Re: RGB Extension of Analyze Particle?

Posted by Gabriel Landini on
URL: http://imagej.273.s1.nabble.com/RGB-Extension-of-Analyze-Particle-tp3700300p3700301.html

On Tuesday 20 February 2007 13:04:22 Claude Bonnard wrote:
> -From an RGB image, select one color and define from it a mask by
> thresholding or other mode of selection. This "Mask" image would contain
> binary objects that could be analyzed as described in ImageJ decumentation.
> -Instead of determining the values for the selected color (Area, Mean etc)
> for each particle defined this way, what I would like is to be able to
> determin, for each particle the values in the R, G and B channels. The mask
> would be here to create those multiple ROI applying to every color (not a
> Gray-valoe replacing the colors).

Use some method (colour thresholding?) to get your binary image holding the
particles.
Split the rgb image into 3 planes (R, G & B)
Use the binary image with the Particle Analyzer (or Particles8_Plus) and
use "redirect" to the R image.
Repeat the last step for the G and B images.

Now you should have the greyscale statistics for each image plane which you
could merge using some spreadsheet program.

G.