gray-color merge

Posted by Knecht, David on
URL: http://imagej.273.s1.nabble.com/gray-color-merge-tp3700357.html

I have two stacks which I would like to merge. One is the grayscale  
of the DIC image and the other is a grayscale image with a color LUT  
applied.  It needs to come as color on gray and would be best if the  
color were subtracted from the gray prior to merge so that the color  
is not washed out by the gray.   The only way I have found is to  
convert the 8 bit LUT image to RGB, split the RGB, then subtract each  
from the gray channel, then use RGB Gray Merge to merge them into a  
new RGB.  It works, but perhaps there is a better way?  Dave

Dr. David Knecht
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)