Overlay with Image5D

Posted by Florian Blaschke on
URL: http://imagej.273.s1.nabble.com/no-subject-tp3700530p3700533.html

Hi Justin,

thanks a lot for your help! Following your recommendation I used Image5D
and I had no problems to colorize the pictures. However, I am struggling
with the overlay. How do you open the two files as two different channels
and how do you finally do the overlay.
There was no reason to use FITC over DIC.

Florian

> Hi,
> I have DAPI/DIC images that I like to colorize and merge.  I was using
> the color merge plugin, but I find that Image5D
> http://rsb.info.nih.gov/ij/plugins/image5d.html is rather convenient
> and it's easier to pick the colors you want.
>
> You need to open the two files as two different channels.  Then select
> overlay in the drop down box (I think it actually says ovly or
> something like that).  Then you simply select a channel and pick a
> color.  The plugin does some other nice stuff as well.
>
> Is there any reason to use FITC over DIC?
>
> Justin
>