Posted by
Karsten Rodenacker on
Jan 19, 2007; 8:23am
URL: http://imagej.273.s1.nabble.com/Segmentation-troubleshooting-tp3700540p3700544.html
Hi,
you show interesting examples.
Allow me some remarks:
1. Seemingly you have used images which were originally stored in
jpeg format with relatively low quality. I think you should improve
the quality considerably if the vessel structure is of interest.
2. Vessel structure of the control seems similar to the vessel
structure in the treated case INSIDE some lets call it hot (cold?)
regions or patches.
3. For characterizing structures never use mosaics from beginning!
For differentiation of tumour and non tumor regions it should be
clear which clues should be used or are of importance e.g.: micro-
structure(texture), macro structure and/or isolated events ...:
I recommend math morphology: (see plugin graymorphology)
microstructure: grayscale tophat transformation radius 2, circular):
original minus open(radius 2); threshold
macrostructure: vessel free or vessel rare zones: inversion of
closure (radius 4-5, circular) of the microstructure; threshold
isolated events: difficult in control, bright spots in treated.
Seemingly in control there are spots like in treated, slightly
larger, but with finer vessel structure.
Good luck,
KR
Am 19.01.2007 um 01:10 schrieb Allison Hall:
> Image J to quantify functional fluorescently
Karsten Rodenacker
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