use of imageJ for herbivory assessment

Posted by Jonathan Rosenthal on Jan 15, 2007; 6:49am
URL: http://imagej.273.s1.nabble.com/use-of-imageJ-for-herbivory-assessment-tp3700587.html

Hello all,
    I'm attempting to use ImageJ to analyze scanned images of leaves in
order to assess percentage of leaf area lost to herbivory.  In so
doing, I've encountered two problems already (and I've just started):
1) The "wand" tool, selected from the toolbar, will, at most outline
only one hole per leaf, will not outline the leaf perimeter, and, more
generally, is often completely non-responsive; and 2) in trying to use
a mouse or trackpoint to control the "freehand" tool from the toolbar
to outline the leaf perimeter (or reconstruct an eaten leaf perimeter),
my hand clearly is not steady enough to closely follow the path that I
desire.     I would greatly appreciate it if anyone would suggest
either alternatives to these 2 tools, or ways that I can use them more
effectively.  Also, if you know of a standard protocol for herbivory
assessment that's compatible with the current version of ImageJ, that
would probably solve all of my problems.
          Thanks very much,
          Jonathan Rosenthal