Re: sperm head area

Posted by Martin Wessendorf on Jan 04, 2007; 6:13pm
URL: http://imagej.273.s1.nabble.com/sperm-head-area-tp3700677p3700678.html

Somporn Duanyai wrote:

> Please advise me how to use Image j for measuring sperm head area.

What do you mean by the "sperm head area"?  The surface area?  Or the
cross-sectional area?

In either case, the answer that you obtain may depend as much on optical
and stereological issues as it does on the specific method you use.
Different species have different sized sperm heads; some are so small
that it could be challenging to obtain an accurate measurement by
optical microscopy.

The stereological issues are outlined in CV Howard and MG Reed's book,
"Unbiased Stereology".  If you haven't already, consult that or a
similar book before you go too much further.

Good luck!

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455             E-mail: martinw[at]med.umn.edu