Re: Stereology with Image J?

Posted by David Finkelstein-2 on Jan 04, 2007; 8:10pm
URL: http://imagej.273.s1.nabble.com/sperm-head-area-tp3700677p3700679.html

Hi Image J ers,
Are there any Stereology plug ins for Image J.
Specifically, an ability to drop\superimpose a counting frame (of user
defined size and step size) onto a section image.  Then the ability to zoom
into each of the counting frames in order.
Regards
David

David Finkelstein (PhD),
The Mental Health Research Institute of Victoria,
155 Oak Street, Parkville, Victoria 3052
AUSTRALIA
-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Martin
Wessendorf
Sent: Friday, 5 January 2007 5:14 AM
To: [hidden email]
Subject: Re: sperm head area

Somporn Duanyai wrote:

> Please advise me how to use Image j for measuring sperm head area.

What do you mean by the "sperm head area"?  The surface area?  Or the
cross-sectional area?

In either case, the answer that you obtain may depend as much on optical
and stereological issues as it does on the specific method you use.
Different species have different sized sperm heads; some are so small
that it could be challenging to obtain an accurate measurement by
optical microscopy.

The stereological issues are outlined in CV Howard and MG Reed's book,
"Unbiased Stereology".  If you haven't already, consult that or a
similar book before you go too much further.

Good luck!

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455             E-mail: martinw[at]med.umn.edu