Re: Stereology with Image J?

Posted by Jacqueline Ross on Jan 05, 2007; 12:04am
URL: http://imagej.273.s1.nabble.com/sperm-head-area-tp3700677p3700681.html

Thanks very much Martin,

I'll have a look for the references you mentioned. I used to have a
rather good old stereology book when I was in Australia that was really
useful but unfortunately I didn't bring it back with me. I seem to
remember having to keep on sampling until the CV was below 5%...

Cheers,

Jacqui

Jacqueline Ross
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: [phone] Ext 87438
Fax: [phone]

http://www.health.auckland.ac.nz/biru/ 

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of
Martin Wessendorf
Sent: 05 January 2007 12:29
To: [hidden email]
Subject: Re: Stereology with Image J?

Jacqui Ross wrote:

> Since Martin has mentioned the Grid plugin which works well for
> systematic sampling, can anyone remind me how you designate the size
of
> the grid relative to your image field that you should use for sampling
> for manual counting, etc.?

When you open the plugin, you get prompted to enter the values that you
want.  With regard to choosing the appropriate values--that'll depend
upon the variability in your system: the more variability within your
sections, the more grids you'd want to count per section.  The Howard
and Reed stereology book goes over this well; also there was a wonderful

little article by John Basgen in Microscopy Today that covered this,
sometime in the past two years.

Martin
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455             E-mail: martinw[at]med.umn.edu