Re: Stereology with Image J?

Posted by Martin Wessendorf on Jan 04, 2007; 11:29pm
URL: http://imagej.273.s1.nabble.com/sperm-head-area-tp3700677p3700684.html

Jacqui Ross wrote:

> Since Martin has mentioned the Grid plugin which works well for
> systematic sampling, can anyone remind me how you designate the size of
> the grid relative to your image field that you should use for sampling
> for manual counting, etc.?

When you open the plugin, you get prompted to enter the values that you
want.  With regard to choosing the appropriate values--that'll depend
upon the variability in your system: the more variability within your
sections, the more grids you'd want to count per section.  The Howard
and Reed stereology book goes over this well; also there was a wonderful
little article by John Basgen in Microscopy Today that covered this,
sometime in the past two years.

Martin
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455             E-mail: martinw[at]med.umn.edu