Re: Stereology with Image J?

Posted by Martin Wessendorf on Jan 04, 2007; 8:31pm
URL: http://imagej.273.s1.nabble.com/sperm-head-area-tp3700677p3700685.html

David Finkelstein wrote:

> Are there any Stereology plug ins for Image J.
> Specifically, an ability to drop\superimpose a counting frame (of user
> defined size and step size) onto a section image.  Then the ability to zoom
> into each of the counting frames in order.

There's the "grid" plugin ("Draw line or point grids":
http://rsb.info.nih.gov/ij/plugins/grid.html).  It's good for randomly
creating a grid over an image, e.g. for cavalieri estimations of volume
or to create a random set of points for systematic random sampling.

For a counting frame, we simply draw one on a piece of acetate that we
tape to the computer monitor.  Very low-tech but it works.

The "IJStage and IJSerial (microscope stage control and serial I/O)"
plugin (http://www.eslide.net/ijstage.php) appears able to control some
types of stages but you'd need to program in the specifics for
systematic random sampling.

Good luck--

Martin Wessendorf

--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
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