Re: sperm head area

Posted by dksamuel on Jan 05, 2007; 3:55am
URL: http://imagej.273.s1.nabble.com/sperm-head-area-tp3700677p3700686.html

How much contrast will be there between the head and tail,Is it a stained
colored image (photo) or a live preview, anyway you have to define an area
as head is it circular and uniform sized, How many particles you plan to
measure, and what measurements, only length or perimeter or volume or all !,
please clarify, regards Samuel

On 1/4/07, Martin Wessendorf <[hidden email]> wrote:

>
> Somporn Duanyai wrote:
>
> > Please advise me how to use Image j for measuring sperm head area.
>
> What do you mean by the "sperm head area"?  The surface area?  Or the
> cross-sectional area?
>
> In either case, the answer that you obtain may depend as much on optical
> and stereological issues as it does on the specific method you use.
> Different species have different sized sperm heads; some are so small
> that it could be challenging to obtain an accurate measurement by
> optical microscopy.
>
> The stereological issues are outlined in CV Howard and MG Reed's book,
> "Unbiased Stereology".  If you haven't already, consult that or a
> similar book before you go too much further.
>
> Good luck!
>
> Martin Wessendorf
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455             E-mail: martinw[at]med.umn.edu
>