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where can I find a theoretical PSF image of two-photon laser scanning microscopy

Posted by hmzhang on Jan 04, 2007; 1:10am
URL: http://imagej.273.s1.nabble.com/where-can-I-find-a-theoretical-PSF-image-of-two-photon-laser-scanning-microscopy-tp3700718.html

Dear friends,

I have some stacks of in vivo two-photon fluorescence images of olfactory bulb, and I want to restored more details of neuronal structure via image restoration algorithms such as iterative constrained Tikhonov-Miller (ICTM),Richarson-Lucy deconvolution,Blind Deconvolution(BD),et al.However, the image of PSF is not available either theoretically or experimentally, and I could not obtain better result.So where can I find a theoretical PSF image of two-photon laser scanning microscopy?
I appreciate your comments or suggestions on this subject.


thanks in advance,
Hongmin

[hidden email]
2007-01-04

       
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