Posted by
lechristophe on
Dec 22, 2006; 9:46am
URL: http://imagej.273.s1.nabble.com/Colocalization-tp3700744p3700745.html
There's a paper, even better a "tutorial review" by Fabrice Cordelières
(author of several excellent ImageJ plugins and eminent member of our
community) in the last issue of Journal of Microscopy :
A guided tour into subcellular colocalization analysis in light microscopy
S. BOLTE, F. P. CORDELIÈRES
http://www.blackwell-synergy.com/doi/abs/10.1111/j.1365-2818.2006.01706.x?ai=sp&ui=2b12f&af=HIt introduces JaCoP (Just another Colocalization Plugin) which proposes
several methods for assessing colocalization.
Hope this helps,
Christophe Leterrier
Antje wrote:
> Hello,
>
> I was playing around with the ICA (Intensity Correlation Analysis) of
> the WCIF bundle.
> I've analyzed images of cells taken with a confocal microscope.
> Now, I've seen that the resulting vePDM-values differ if I choose only
> one cell or analyze the whole image. I guess this has something to do
> with the calculation of the "mean" signal, which then might differ.
> But, I'm afraid that this value also differs, depending on the density
> of cells... (what would screw up my analysis)
> Does anybody have an idea how to handle this? Is there anybody, who did
> serious colocalization analysis of a larger amount of data?
>
>
> Antje
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