Posted by
Itsaso Garcia on
Dec 21, 2006; 3:44pm
URL: http://imagej.273.s1.nabble.com/Colocalization-tp3700744p3700746.html
Hi!
I also make colocalization analysis in confocal micrographies, and i
use the plugin "colocalization thresholds". After learning a little bit
about the colocalization describing parameters, i think one can obtain
a lot of information with this plugin. I don´t know about ICA, though.
I would reccomend you to read the papers by: Costes SV et al, Biophys J
2004; 86(6): 3993-4003; and Manders E. et al, Journal of Microscopy
1993; 169:375-382.
I hope this helps, good luck!
Itsaso Garcia-Arcos
P.S: Happy Christmas and New Year to everyone!
> Hello,
>
> I was playing around with the ICA (Intensity Correlation Analysis) of
> the WCIF bundle.
> I've analyzed images of cells taken with a confocal microscope.
> Now, I've seen that the resulting vePDM-values differ if I choose only
> one cell or analyze the whole image. I guess this has something to do
> with the calculation of the "mean" signal, which then might differ.
> But, I'm afraid that this value also differs, depending on the density
> of cells... (what would screw up my analysis)
> Does anybody have an idea how to handle this? Is there anybody, who
did
> serious colocalization analysis of a larger amount of data?
>
>
> Antje
>
>
>
>
>
>
>
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--
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Itsaso Garcia-Arcos
Department of Physiology, Medical School,
University of the Basque Country. UPV/EHU
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