ImageJ Related Question About Software

Posted by ev34 on Dec 13, 2006; 8:22pm
URL: http://imagej.273.s1.nabble.com/ImageJ-Related-Question-About-Software-tp3700828.html

Hi,

Could anyone recommend a good, (preferrably freeware) software to
capture microscope images? I am mostly interested in capturing
fluorescence in my images, using a bandwith filter in the low to mid 500
nm. The microscope I use is the Nikon inverted Eclipse TE2000-S, the
camera is a relatively old, automatic Cool Snap brand, so I need to be
sure that the software is compatible with both of these. I have been
using the Roper Scientific software and have been having quite a few
problems with the settings, especially with getting a sharp, focused
image (sometimes I get a completely black screen showing up without any
image for no apparent reason). Because I do not have a lot of
fluorescence in my specimens, I need something that could be configured
to be quite sensitive. I use ImageJ to analyze the fluorescence.

Thanks!

Lena