measurement of axons diameter and area

Posted by Yu, K.L. on Nov 28, 2006; 8:47pm
URL: http://imagej.273.s1.nabble.com/measurement-of-axons-diameter-and-area-tp3700920.html

Dear all,

Does anyone of you have experience measuring the diameter and area of myelinated axons of the spinal ventral roots? They were ultrathin sections (1um), stained with Toluidine blue and observed in light microscope. Is there an efficient way to measure them in an automatic scale?  I have problems to adjust the background threshold. I appreciate very much if you could share your protocol or opinions in the processing via ImageJ.

Thanks in advance.

Yours sincerely
Carol Yu