Re: measurement of axons diameter and area

Posted by Raad Nashmi on Nov 30, 2006; 5:17am
URL: http://imagej.273.s1.nabble.com/measurement-of-axons-diameter-and-area-tp3700920p3700921.html

Dear Carol,

You may want to overlay a grid over your image of axons.  Then you
would analyzed all the axons that touches the lines of the
grid.  This would be a non-biased sampling method of measuring axonal diameter.

You may also want to measure not only axonal diameter excluding
myelin (d) but also the diameter of the axonal fiber including the
myelin (D).  The myelination ratio (MR) also gives important
information (MR=D/d).
The methodology is published in Nashmi and Fehlings 2001 Neuroscience
104:235-251.

Regards,
Raad Nashmi

At 12:47 PM 11/28/2006, you wrote:

>Dear all,
>
>Does anyone of you have experience measuring the diameter and area
>of myelinated axons of the spinal ventral roots? They were ultrathin
>sections (1um), stained with Toluidine blue and observed in light
>microscope. Is there an efficient way to measure them in an
>automatic scale?  I have problems to adjust the background
>threshold. I appreciate very much if you could share your protocol
>or opinions in the processing via ImageJ.
>
>Thanks in advance.
>
>Yours sincerely
>Carol Yu

-------------------------------------------
Raad Nashmi   PhD
Senior Research Fellow - Lester Lab
California Institute of Technology
Division of Biology, M/C156-29
1200 E. California Blvd
Pasadena, CA, 91125
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