Re: measurement of axons diameter and area

Posted by Dimiter Prodanov on Nov 29, 2006; 10:00am
URL: http://imagej.273.s1.nabble.com/measurement-of-axons-diameter-and-area-tp3700920p3700922.html

Hi Carol,

I did mostly automatic measurements of axons. As far as I know
there is no satisfactory automatic routine for identification of the
whole fiber =
myelin sheath + axon. Few days ago someone proposed using snakes,
"active contrours"
so you can try that.
Sampling is indeed very important, but I dont agree that 100-200 fibers
is enough for large nerves.
In my experience per nerve section you should sample 25 - 40 % of the
area.
You can significantly reduce the brightness artifact by taking a mean
filter of say 25
and then subtracting the original image from the filtered one, just make
sure you tick
the 32-bit option of the Image Calculator, and then you can set the
Brightness automatically and
go back to 8-bit image.

best regards

Dimiter Prodanov