Counting blood cells on a hemocytometer

Posted by Gregor F Barclay on Nov 28, 2006; 3:58pm
URL: http://imagej.273.s1.nabble.com/Counting-blood-cells-on-a-hemocytometer-tp3700937.html

I need a protocol for using Image J to count blood cells on a
hemocytometer. I have hundreds of counts to make, and I thought that IJ
would make this easy, but I cannot seem to find a way to threshold out
or otherwise make the grid lines insignificant enough that IJ sees just
the cells.

 

Any suggestions, especially  from someone who has done this, would be
appreciated. I can use Bright Field, Phase Contrast, DIC, or
epifluorescence microscopy to view the blood cells.

 

Greg Barclay

 

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We make a living by what we get, we make a life by what we give. - Sir
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Dr. G. F. Barclay
Department of Life Sciences
University of the West Indies
St. Augustine
Republic of Trinidad and Tobago
868-662-2002 ext 3112