Re: Counting blood cells on a hemocytometer

Posted by lechristophe on Nov 28, 2006; 4:04pm
URL: http://imagej.273.s1.nabble.com/Counting-blood-cells-on-a-hemocytometer-tp3700937p3700939.html

If you can have both bright field and fluo, maybe you can define the
area with the brightfield image (where you see the lines) and report
this ROI on the fluo image (where I assume you just see the cells), then
count inside this ROI.

Just a suggestion

Christophe

Gregor F Barclay wrote:

> I need a protocol for using Image J to count blood cells on a
> hemocytometer. I have hundreds of counts to make, and I thought that IJ
> would make this easy, but I cannot seem to find a way to threshold out
> or otherwise make the grid lines insignificant enough that IJ sees just
> the cells.
>
>  
>
> Any suggestions, especially  from someone who has done this, would be
> appreciated. I can use Bright Field, Phase Contrast, DIC, or
> epifluorescence microscopy to view the blood cells.
>
>  
>
> Greg Barclay
>
>  
>
> [hidden email]
>
>