Re: Substract diffuse reflection.... #2

Posted by Liisa Palomaa on
URL: http://imagej.273.s1.nabble.com/Substract-diffuse-reflection-tp3701147p3701155.html

Hi...

Sorry.... I was a little bit unclear before.

The line I want to substract is a line- that I select with the
"straight line selection tool"....... So it doesn't have a constant
greyscale value. The selection should be at the position x=192,y=0 and
x=192, y=192)Then I do a plot profile and use a median filter to
smooth the plot profile. After that I want to create a new image with
a radius that is the same as the line (192 pixels) and have the same
light intesity as the line. So the circle should be dark in the midle.
 The last step is just to substract the circle image from the original
image.

//Liisa