Stack Focuser

Posted by NeLaS on
URL: http://imagej.273.s1.nabble.com/Stack-Focuser-tp3701292.html

Hello,

I have a series of light microscopy photos with different focal planes,
taken under DIC. I was trying to create a focused composition image of this
photos, but I'm not getting very good images.

I'm creating a stack with the pictures and using the Stack Focuser plugin.
If I use a small n (for the n x n kernel) like 3, I get the image in focus
but with lots of blurring. If I use a bigger n (like 100) I get a better
definition of the focused parts but the squares of the filter are very
obvious, and some of them are not in focus at all!

I know this images have a lot of unfocused noise which might be the
responsible for the low quality result, but any suggestions in how to get
focused stacks are very welcome!

Is there any other tool/plugin I can use to do this?

Thanks in advance!

Bruno

--
Centro de Biologia Marinha (CEBIMar)
Universidade de São Paulo
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11600-000 São Sebastião, SP, Brasil

www.myspace.com/nelas