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Re: point selection and counting procedure

Posted by Robert Baer on Oct 16, 2006; 2:42pm
URL: http://imagej.273.s1.nabble.com/point-selection-and-counting-procedure-tp3701294p3701295.html

You may find Kurt De Vos's cell-counter plugin
(http://rsb.info.nih.gov/ij/plugins/cell-counter.html) helpful for what you
are trying to do.  It gives you the possibility of counting and marking
multiple types of objects in an image.

HTH,
Rob


----- Original Message -----
From: "Thorsen, Anders" <[hidden email]>
To: <[hidden email]>
Sent: Monday, October 16, 2006 5:53 AM
Subject: point selection and counting procedure


> Hi,
>
> We are counting oocytes from whole mount pictures of fish ovaries. We are
> counting a lot of samples so our counting procedure should be as efficient
> as possible. The majority of the oocytes (the dark vitellogenic ones) are
> counted easily using the analyze particle function. However, after the
> particle analysis has been run there is still some oocytes that have not
> been counted. Mainly this is the previtellogenic oocytes that are
> transparent and difficult to threshold on. These oocytes have to be
> counted
> manually on the picture. I find using the point selection tool is in many
> cases efficient, however we should only count oocytes that are larger than
> 150 µm. That means that oocytes around this threshold value has to be
> measured using the line tool to decide wether it should be counted or not.
> Since this is a new type of measurement ImageJ then want to empty the
> results window to make room for the new type of data, and this makes it
> very
> cumbersome. We could of course do all the manual counting using the line
> tool, but that would take much more time and we have a lot of samples.
>
> I can think of two solutions to this: 1. Make ImageJ accept several types
> of
> measurements in the Results window at the same time. 2. Let the point
> selection tool (the cross) have a selectable size so that it can function
> as
> a size reference when doing the point selection. I have looked for plugins
> or macros that could help us with this but haven't found any. Is anybody
> aware of something that could solve this problem? Is there somthing I am
> doing wrong? Could a plugin be made to solve this?
>
> Background:
> We are an ICES working group that is responsible for making egg-production
> estimates for mackerel and horse-mackerel in the North Sea and Norwegian
> Sea
> from mature pre-spawning ovaries. The egg-production estimates are then
> compared to the amount of pelagic eggs of these species that is actually
> found (using plankton net) during the spawning season. From this the total
> spawner biomass is calculated, and this again makes an important basis for
> deciding the fishing quotas for these species.
>
> thank you
>
> anders
>
> ..................................
> Anders Thorsen
> Dr. Scient.
> Senior Scientist
> Recruitment Biology and Behaviour
> Institute of Marine Research
> Nordnesgaten 50
> P. O. Box 1870 Nordnes
> N-5817 Bergen, Norway
> Phone: +47 55238444
> Fax: +47 55238531
> E-mail: [hidden email]
>