Posted by
Rob Lee-2 on
Oct 12, 2006; 4:44pm
URL: http://imagej.273.s1.nabble.com/ImageJ-Movie-of-simultaneous-images-and-plotting-tp3701317.html
Hi.
I'm using ImageJ to make movies of Ca2+ dynamics in fura-2-loaded cells.
Basically we're importing the time-lapse images from Perkin Elmer Ultraview
software, making them into stacks and going from there. What we'd love to do
is to be able to show an movie with the cell/Ca2+ pics and an animation of
simultaneous generation of a plot/graph of the fura2 ratio-derived Ca2+
concentration vs. time. I've seen this done in several presentations, but am
now sure how people do it. Can anyone give me any suggestions as to how to
approach this problem?
One way to do it would be to generate a stack of images of the plot (each one
with an additional time point) and then combine that with the stack of Ca
images and make a movie of the two images together, but generating hundreds of
graph images for each long experimen seems like a daunting amount of work.
We're doing most of our plotting in Igor Pro, so it may be possible to generate
an Igor macro to produce a graph for each time point which could then be
imported as a stack into ImageJ. However, I was wondering if anyone had any
better suggestions. I appreciate any suggestions and I apologize if this
overlaps with previous discussions--I'm new to this list.
thanks,
rob
--
Rob Lee
CAMB Graduate Student
Foskett Lab
Dept of Physiology
Univ. of Penn. School of Medicine
[hidden email]
215-898-0468 (lab)
724-493-7110 (cell)