http://imagej.273.s1.nabble.com/ImageJ-Movie-of-simultaneous-images-and-plotting-tp3701317p3701318.html
> Hi.
>
> I'm using ImageJ to make movies of Ca2+ dynamics in fura-2-loaded
> cells.
> Basically we're importing the time-lapse images from Perkin Elmer
> Ultraview
> software, making them into stacks and going from there. What we'd
> love to do
> is to be able to show an movie with the cell/Ca2+ pics and an
> animation of
> simultaneous generation of a plot/graph of the fura2 ratio-derived Ca2+
> concentration vs. time. I've seen this done in several presentations,
> but am
> now sure how people do it. Can anyone give me any suggestions as to
> how to
> approach this problem?
>
> One way to do it would be to generate a stack of images of the plot
> (each one
> with an additional time point) and then combine that with the stack of
> Ca
> images and make a movie of the two images together, but generating
> hundreds of
> graph images for each long experimen seems like a daunting amount of
> work.
> We're doing most of our plotting in Igor Pro, so it may be possible to
> generate
> an Igor macro to produce a graph for each time point which could then
> be
> imported as a stack into ImageJ. However, I was wondering if anyone
> had any
> better suggestions. I appreciate any suggestions and I apologize if
> this
> overlaps with previous discussions--I'm new to this list.
>
> thanks,
>
> rob
>
>
>
>
> --
> Rob Lee
> CAMB Graduate Student
> Foskett Lab
> Dept of Physiology
> Univ. of Penn. School of Medicine
>
[hidden email]
> 215-898-0468 (lab)
> 724-493-7110 (cell)
>