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Re: ImageJ: Movie of simultaneous images and plotting

Posted by Knecht, David on Oct 13, 2006; 3:03am
URL: http://imagej.273.s1.nabble.com/ImageJ-Movie-of-simultaneous-images-and-plotting-tp3701317p3701319.html

I did one sort of like this a while back.  I made a movie of the  
cells in quicktime.  Then I made a graph of the data as a tiff file  
from Prism or Excel.  I brought both into Flash and made a dot that  
followed the path of the tiff graph as a path animation in the same  
timeline as the quicktime movie was playing.  That was then exported  
as a quicktime movie.  Dave

Dr. David Knecht
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)


On Oct 12, 2006, at 1:52 PM, Wayne Rasband wrote:

> You can do this by using the StackProfilePlot macro at
>
>     http://rsb.info.nih.gov/ij/macros/StackProfilePlot.txt
>
> to generate a stack of profile plots and then using the  
> Stack_Combiner plugin at
>
>      http://rsb.info.nih.gov/ij/plugins/combiner.html
>
> to combine the stack of profile plots with the source stack.
>
> -wayne
>
> On Oct 12, 2006, at 11:44 AM, Rob Lee wrote:
>
>> Hi.
>>
>> I'm using ImageJ to make movies of Ca2+ dynamics in fura-2-loaded  
>> cells.
>> Basically we're importing the time-lapse images from Perkin Elmer  
>> Ultraview
>> software, making them into stacks and going from there.  What we'd  
>> love to do
>> is to be able to show an movie with the cell/Ca2+ pics and an  
>> animation of
>> simultaneous generation of a plot/graph of the fura2 ratio-derived  
>> Ca2+
>> concentration vs. time.  I've seen this done in several  
>> presentations, but am
>> now sure how people do it.  Can anyone give me any suggestions as  
>> to how to
>> approach this problem?
>>
>> One way to do it would be to generate a stack of images of the  
>> plot (each one
>> with an additional time point) and then combine that with the  
>> stack of Ca
>> images and make a movie of the two images together, but generating  
>> hundreds of
>> graph images for each long experimen seems like a daunting amount  
>> of work.
>> We're doing most of our plotting in Igor Pro, so it may be  
>> possible to generate
>> an Igor macro to produce a graph for each time point which could  
>> then be
>> imported as a stack into ImageJ.  However, I was wondering if  
>> anyone had any
>> better suggestions.  I appreciate any suggestions and I apologize  
>> if this
>> overlaps with previous discussions--I'm new to this list.
>>
>> thanks,
>>
>> rob
>>
>>
>>
>>
>> --
>> Rob Lee
>> CAMB Graduate Student
>> Foskett Lab
>> Dept of Physiology
>> Univ. of Penn. School of Medicine
>> [hidden email]
>> 215-898-0468 (lab)
>> 724-493-7110 (cell)
>>