http://imagej.273.s1.nabble.com/ImageJ-Movie-of-simultaneous-images-and-plotting-tp3701317p3701319.html
I did one sort of like this a while back. I made a movie of the
cells in quicktime. Then I made a graph of the data as a tiff file
from Prism or Excel. I brought both into Flash and made a dot that
timeline as the quicktime movie was playing. That was then exported
as a quicktime movie. Dave
Dr. David Knecht
91 N. Eagleville Rd.
> You can do this by using the StackProfilePlot macro at
>
>
http://rsb.info.nih.gov/ij/macros/StackProfilePlot.txt>
> to generate a stack of profile plots and then using the
> Stack_Combiner plugin at
>
>
http://rsb.info.nih.gov/ij/plugins/combiner.html>
> to combine the stack of profile plots with the source stack.
>
> -wayne
>
> On Oct 12, 2006, at 11:44 AM, Rob Lee wrote:
>
>> Hi.
>>
>> I'm using ImageJ to make movies of Ca2+ dynamics in fura-2-loaded
>> cells.
>> Basically we're importing the time-lapse images from Perkin Elmer
>> Ultraview
>> software, making them into stacks and going from there. What we'd
>> love to do
>> is to be able to show an movie with the cell/Ca2+ pics and an
>> animation of
>> simultaneous generation of a plot/graph of the fura2 ratio-derived
>> Ca2+
>> concentration vs. time. I've seen this done in several
>> presentations, but am
>> now sure how people do it. Can anyone give me any suggestions as
>> to how to
>> approach this problem?
>>
>> One way to do it would be to generate a stack of images of the
>> plot (each one
>> with an additional time point) and then combine that with the
>> stack of Ca
>> images and make a movie of the two images together, but generating
>> hundreds of
>> graph images for each long experimen seems like a daunting amount
>> of work.
>> We're doing most of our plotting in Igor Pro, so it may be
>> possible to generate
>> an Igor macro to produce a graph for each time point which could
>> then be
>> imported as a stack into ImageJ. However, I was wondering if
>> anyone had any
>> better suggestions. I appreciate any suggestions and I apologize
>> if this
>> overlaps with previous discussions--I'm new to this list.
>>
>> thanks,
>>
>> rob
>>
>>
>>
>>
>> --
>> Rob Lee
>> CAMB Graduate Student
>> Foskett Lab
>> Dept of Physiology
>> Univ. of Penn. School of Medicine
>>
[hidden email]
>> 215-898-0468 (lab)
>> 724-493-7110 (cell)
>>