More on pixel shifting

Posted by Knecht, David on Oct 02, 2006; 3:01pm
URL: http://imagej.273.s1.nabble.com/More-on-pixel-shifting-tp3701425.html

I am still trying to understand pixel shifting.  I came across this  
old post by Jason Kirk when this came up before:

"The Leica DM-IR/BE is an infinity corrected system, therefore an  
emission filter being slightly off-camber in the filter wheel would  
not effect the optical path since light passing the filters is  
parallel.  The most probable reason for seeing a shift AFTER the  
dichroic in the emission path is not the barrier filter, but is if  
the tube lens (recombining element) is somehow off axis. This is the  
only lens element remaining. "
The implication of Jason's post (and no one seems to have  
contradicted him) is that one should not see pixel shifting in a  
properly aligned infinity corrected microscope due to filters.  I  
then queried Semrock about why they would sell Zero Pixel Shift  
filters if this were indeed the case.  They essentially disagreed as  
to the effect of infinity systems on pixel shifting.  Can someone  
clarify this issue?  THanks- Dave


Dr. David Knecht
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)