(no subject)

Posted by Lhuissier, Franck on
URL: http://imagej.273.s1.nabble.com/no-subject-tp3701656.html

Dear Group,
I've been following this group with great interest for a long time but never actually contributed to it in any way.
Recently though, I ran into a problem that I cannot seem to solve with my favorite image analysis program Object Image and I was wondering if there was an easy way to do that in ImageJ. I'm no Java programmer (that's why I used Object Image, I still have some remnants of my college Pascal), therefore be gentle, this is all new to me.
Thanks in advance for any advice and here is my problem:
I have images of GFP and RFP fluorescing seeds. Some seeds fluoresce green, some red and some both.
I've taken black and white images of the same field of view for the two wavelengths. I have now several red images and several green images labeled as follow
GFP-001
GFP-002
GFP-003
etc...
RFP-001
RFP-002
RFP-003
etc...
GFP-001 matching of course RFP-001.
What I would like to do is to check into a given directory (selected by the user) the presence of the fluorescent pictures, open the matching pairs, do couple of arithmetic operations to check which seeds are red, green or both, do a particle analysis on the images to end up with a result table containing the number of green, red and red/green seeds among the total amount of seeds in the image, and repeat this for all images in the directory.
I hope I was clear enough.
Again thank you in advance for any help
Regards
Franck
 
Dr. F. Lhuissier
Wageningen University/Keygene
The Netherland