Colocalize, huh?

Posted by Perry, Griffin M on Sep 04, 2006; 9:16pm
URL: http://imagej.273.s1.nabble.com/Colocalize-huh-tp3701672.html

Dear Listserv Users,

 

I have taken z-stacks of nerve tissue and am trying to quantify via
colocalization certain receptors in the nerve fibers.  With the WCIF
ImageJ bundle, there are many colocalization plugins but not very many
help files.  Can anyone help me try and find a solution?  

 

I just want to find the area of the nerve fiber above a certain
threshold and the percentage of the receptor that is colocalized on the
nerve fiber.

 

Colocalization Finder was updated last week, but with the new version, I
don't understand where I can set the threshold; it automatically
thresholds the image.  Can you manually threshold the image?  And what
is the ratio all about?  Which ratio?

 

All plugins are not created equally.  Which one can help us in our
quest?

 

Thanks,

 

Griffin Perry and Lisa Alvarado, DDS

UTHSCSA