Posted by
Sarwar, Rizwan on
Aug 17, 2006; 5:15pm
URL: http://imagej.273.s1.nabble.com/densitometry-tp3701731p3701744.html
Jonathan
Can I please also have a copy too for SDS-PAGE analysis
Tx
Rizwan Sarwar
Physiological Genomics & Medicine
Imperial College London
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
Jonathan Hilmer
Sent: 16 August 2006 21:37
To:
[hidden email]
Subject: Re: densitometry
I have written a guide for ImageJ densitometry, but it isn't really a
straightforward how-to type manual. The method also relies upon
outside software (Photoshop or an equivalent) for convenience, uses
modified ImageJ GelAnalyzer code, and assumes the user has a decent
background in processing images in ImageJ. The benefit from these
inconveniences is that the final result is more accurate than other
methods.
Jonathan Hilmer
Bothner Research Group
Montana State University
On 8/16/06, Miguel Valvano <
[hidden email]> wrote:
> I would like to use imageJ for densitometric analysis of western
blots.
> Does anybody have a writtne protocol. I find it confusion using the
> menus in the gel analyzer.
> Thanks
>
>
> Miguel A. Valvano, M.D.
> Professor and Department Chair
> Canada Research Chair in Infectious Diseases and Microbial
> Pathogenesis
> Department of Microbiology and Immunology, University of Western
> Ontario
> London Ontario, N6A 5C1
> voice 519-661-3436; fax 519-661-3499;
http://publish.uwo.ca/~mvalvano> Assistant: Ms. Evelynne Ansara (
[hidden email]);
> 519-661-2111 #81436