Re: densitometry

Posted by Miguel Valvano on Aug 16, 2006; 9:48pm
URL: http://imagej.273.s1.nabble.com/densitometry-tp3701731p3701745.html

Thank you. I would like to give it a try. Could you mind sending me a
copy of your guide?
Thanks
M
 
Miguel A. Valvano, M.D.
Professor and Department Chair
Canada Research Chair in Infectious Diseases and Microbial
Pathogenesis
Department of Microbiology and Immunology, University of Western
Ontario
London Ontario, N6A 5C1
voice 519-661-3436; fax [phone]; http://publish.uwo.ca/~mvalvano
Assistant: Ms. Evelynne Ansara ([hidden email]);
519-661-2111 #81436

>>> [hidden email] 08/16/06 4:37 pm >>>

I have written a guide for ImageJ densitometry, but it isn't really a
straightforward how-to type manual.  The method also relies upon
outside software (Photoshop or an equivalent) for convenience, uses
modified ImageJ GelAnalyzer code, and assumes the user has a decent
background in processing images in ImageJ.  The benefit from these
inconveniences is that the final result is more accurate than other
methods.


Jonathan Hilmer
Bothner Research Group
Montana State University



On 8/16/06, Miguel Valvano <[hidden email]> wrote:
> I would like to use imageJ for densitometric analysis of western
blots.

> Does anybody have a writtne protocol. I find it confusion using the
> menus in the gel analyzer.
> Thanks
>
>
> Miguel A. Valvano, M.D.
> Professor and Department Chair
> Canada Research Chair in Infectious Diseases and Microbial
> Pathogenesis
> Department of Microbiology and Immunology, University of Western
> Ontario
> London Ontario, N6A 5C1
> voice 519-661-3436; fax [phone];

http://publish.uwo.ca/~mvalvano
> Assistant: Ms. Evelynne Ansara ([hidden email]);
> 519-661-2111 #81436