Re: Preserving pixel values in saved image

Posted by Wayne Rasband on Aug 07, 2006; 2:55pm
URL: http://imagej.273.s1.nabble.com/Preserving-pixel-values-in-saved-image-tp3701875p3701876.html

 > I open a raw image (16-bit unsigned) do some treatment (no
 > change to pixel values though) on it and than I save it
 > again as raw. If I again open the image as raw, the pixel
 > values are rescaled, different from the original. Some of
 > them even flow over the 16-bit value limit. Even worse, the
 > scaling is different if I adjust brightness/contrast. I have
 > un-checked option to Scale image, when converting, but the
 > result is the same. Preservation of the pixel values is
 > critical for what I do.

 >I run ImageJ 1.36b on MacBook Pro. Thanks for advice in advance.

16 and 32 bit images saved using File>Save As>Raw Data are in
big-endian byte order unless "Export Raw in Intel Byte Order" is
checked in the Edit>Options>Input/Output dialog box. This option was
added in ImageJ 1.37e.

-wayne