Re: Slope

Posted by Wayne Rasband on
URL: http://imagej.273.s1.nabble.com/grayscale-displays-and-human-vision-tp3702214p3702218.html

> Does anyone know of a plugin for calculating the slope of
> signal intensity over a time series of image data?

Here is how you can calculate the slope:

1. In Analyze>Set Measurements, check "Mean Gray Value" and uncheck all
other options.
2. Run Image>Stacks>Plot Z-axis Profile
3. Right click in the Results window and select "Copy All" ("Copy" in
v1.37) from the drop down menu.
4. Open the Curve Fitter (Analyze>Tools>Curve Fitting).
5. Select the default data set, then paste (ctrl-v).
6. Click "Fit" and the slope is displayed in the Log window as the "b"
parameter.

-wayne