Re: two color live cell imaging- ratio data

Posted by Wayne Rasband on
URL: http://imagej.273.s1.nabble.com/two-color-live-cell-imaging-ratio-data-tp3702347p3702351.html

> A follow up question to my question.  If I do 32 bit math and divide
> one channel by another or by a constant, I get pixel values ranging
> from roughly 0-2 (with three decimal significance).  If I then apply
> the r-g ratio LUT through the Image/color/edit LUT, it applies the LUT
> as if the data is 0-255.  Is it autoscaling the LUT to the dataset?  
> Is there some way of setting the scale and/or determining what values
> were assigned what colors?  I would like different images to have the
> same scaled LUT, but they won't if it is autoscaled.  Thanks- Dave

The 256 entry LUT is scaled to the pixel value range of the image. With
contrast enhanced images, it is scaled to the display range. The two
numbers under the plot in the Image>Adjust>Brightness>Contrast tool are
the lower and upper limits of the display range. You can also get the
display range by calling the getMinAndMax() macro function.

-wayne