Color thresholding

Posted by Craig Oppel on Jun 23, 2006; 3:17pm
URL: http://imagej.273.s1.nabble.com/Color-thresholding-tp3702421.html

Hello All,

I am working on a project that involves microwave extraction of leaves onto
TLC plates to quantify alkaloids in cardinal flower. The final plates have
a yellow background with a red/orange alkaloid positive leaf imprint. I
have tried color thresholding to eliminate the yellow background, and I can
get acceptable results, but I am concerned because I have to make manual
adjustments to the intensity sliders. I have found that if I first convert
the TIFF images of the TLC plates into 8-bit gray scale images and then use
the adjust contrast/brightness auto function I get really good results.
What I am concerned about is I have no idea how the algorithim processes
the picture. I am taking great pains to eliminate bias in my design. Is
there a way to eliminate the yellow background that would ensure that all
images are treated identically?

Thank you
Craig