Re: Question about autofocus

Posted by Vincenzo Della Mea on May 17, 2006; 7:15am
URL: http://imagej.273.s1.nabble.com/Question-about-running-ImageJ-on-a-multi-CPU-workstation-tp3702722p3702727.html

Not directly in ImageJ, however we are finishing a system for whole  
slide acquisition which includes also an autofocus module (described  
in Della Mea V, Viel F, Beltrami CA. A pixel-based autofocusing  
technique for digital histologic and cytologic slides. Computerized  
Medical Imaging and Graphics 2005; 29(5): 333-341.).
It is somewhat easy to do something similar: you need a focus measure  
function (e.g., standard deviation), and some Z-space visit algorithm  
(e.g., linear, or dichotomic). If you do not mind speed, the best  
thing is to scan the z space at the minimum step possible, and keep  
the image with greatest grey level standard deviation. Some more  
adequate focus measures should be used for fluorescent images.

Vincenzo

Il giorno 16/mag/06, alle ore 15:36, Christophe Leterrier ha scritto:

> I'll be very interested to know that, too. I heard they were some  
> serial com plugins to control XY (possibly Z) stages, but I would  
> be very interested to hear about implementing an autofocus function  
> in ImageJ
>
> Christophe Leterrier