Re: Maximum entropy thresholding for 16-bit images

Posted by Sanneke Brinkers on
URL: http://imagej.273.s1.nabble.com/Maximum-entropy-thresholding-for-16-bit-images-tp3702941p3702943.html

I tried the MultiThresholder but got different results with the same images.
Does the plugin use actual 16-bit max entropy thresholding or does it do 1
to 8-bit conversion, but you just can't see it?

 Also, sometimes the thresholder selects the background in stead of the
cells. Did I do something wrong? Or are there more settings I just haven't
found yet? How can I control this plugin better?  

Thanks,

Sanneke

P.S. I will also try to adjust the max entropy plugin with the suggestion
from Johannes Schindelin and see if it fits my needs


-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of Wayne
Rasband
Sent: maandag 1 mei 2006 23:38
To: [hidden email]
Subject: Re: Maximum entropy thresholding for 16-bit images

> The current maximum entropy threshold plugin needs 8-bit images as
> input. However, my images are 16-bit TIFF. When I convert the images
> to 8-bit a lot of information is lost, so my question is: Is there a
> maximum entropy thresholding plugin available for 16-bit images?
>
> Or can the writer of the current plugin give me tips on how to convert
> the plugin, or rewrite it himself to include 16-bit images?

The MultiThresholder plugin at

     http://rsb.info.nih.gov/ij/plugins/multi-thresholder.html

does maximum entropy thresholding on 16-bit images.

-wayne