Straightening a segmented line

Posted by Rafael Carazo-Salas-2 on Apr 24, 2006; 7:06pm
URL: http://imagej.273.s1.nabble.com/Straightening-a-segmented-line-tp3702999.html

Dear NIH Image users ,

I am analyzing 2D images which contain mostly background and some
'fluorescence lines' which represent the signal .  Although the lines are
mostly straight the do bend often and so to analyze them I use the Segmented
Line Selection , followed by the Plot Profile tool .  This gives me the
fluorescence per pixel for the whole segmented line selected and this data
can then be saved as a table .

I would like to do something slightly more sophisticated .  I would like to
:  1) create a segmented line selection , then 2) open a new window as wide
as the total segmented line length , and then 3) 'paste' the fluorescence
intensity information from my segmented line in this new window (in a
nutshell , I want to straighten the segmented line and create a new
straightened line window with the fluorescence information in each pixel of
it) .

If this is not too confusing would any of you have ideas about this ? I have
written a macro fo this but it seems to complicated to be the most efficient
way to accomplish this thing .

Thank you very much in advance !  Cheers ,





Rafael



_____________________________________________________________________

Rafael Edgardo Carazo Salas, Ph.D.
Cell Cycle Laboratory
Cancer Research UK, London Research Institute
44 Lincoln's Inn Fields
London WC2A 3PX, UNITED KINGDOM
Tel ::: [phone]
Fax ::: [phone]
_____________________________________________________________________