Re: Straightening a segmented line

Posted by Liu, Dongfang (NIH/NIAID) [F] on Apr 24, 2006; 8:49pm
URL: http://imagej.273.s1.nabble.com/Straightening-a-segmented-line-tp3702999p3703001.html

could you share your macro with us?
Thank you very much.

Best regards

> ----------
> From: Rafael Carazo-Salas
> Reply To: List IMAGEJ
> Sent: Monday, April 24, 2006 2:06 PM
> To: List IMAGEJ
> Subject: Straightening a segmented line
>
> Dear NIH Image users ,
>
> I am analyzing 2D images which contain mostly background and some
> 'fluorescence lines' which represent the signal .  Although the lines are
> mostly straight the do bend often and so to analyze them I use the Segmented
> Line Selection , followed by the Plot Profile tool .  This gives me the
> fluorescence per pixel for the whole segmented line selected and this data
> can then be saved as a table .
>
> I would like to do something slightly more sophisticated .  I would like to
> :  1) create a segmented line selection , then 2) open a new window as wide
> as the total segmented line length , and then 3) 'paste' the fluorescence
> intensity information from my segmented line in this new window (in a
> nutshell , I want to straighten the segmented line and create a new
> straightened line window with the fluorescence information in each pixel of
> it) .
>
> If this is not too confusing would any of you have ideas about this ? I have
> written a macro fo this but it seems to complicated to be the most efficient
> way to accomplish this thing .
>
> Thank you very much in advance !  Cheers ,
>
>
>
>
>
> Rafael
>
>
>
> _____________________________________________________________________
>
> Rafael Edgardo Carazo Salas, Ph.D.
> Cell Cycle Laboratory
> Cancer Research UK, London Research Institute
> 44 Lincoln's Inn Fields
> London WC2A 3PX, UNITED KINGDOM
> Tel ::: [phone]
> Fax ::: [phone]
> _____________________________________________________________________
>
>