Re: problems converting to 8-bit image

Posted by Robert Baer on Apr 07, 2006; 10:07pm
URL: http://imagej.273.s1.nabble.com/problems-converting-to-8-bit-image-tp3703137p3703140.html

You could try converting to RGB color first, and then to RGB grayscale or
alternaively then using using Image<Color<RGB Split to create three separate
grayscale images one of which should give you the best contrast.

Rob Baer

____________________________
Robert W. Baer, Ph.D.
Associate Professor
Department of Physiology
A. T. Still University of Health Science
800 W. Jefferson St.
Kirksville, MO 63501-1497 USA
----- Original Message -----
From: "Name Suppressed - 000002948" <[hidden email]>
To: <[hidden email]>
Sent: Friday, April 07, 2006 2:29 PM
Subject: problems converting to 8-bit image

> I have quite a few images of plant roots traced in blue and black
> marker. I'm trying to do some fractal analysis and want to convert
> these images to 8-bit grayscale. The images are currently 8-bit color.
> I've tried using 'image->type->8-bit' and that works for some of my
> images, but for others the entire image becomes black. I tried
> thresholding first and that will convert the entire root system to
> black, but when I try converting to 8-bit grayscale the entire image
> still becomes black.  Is there anything I can do about this?
>
> Thanks,
> Kathryn Barto
> Environmental Science PhD Program
> Wright State University
>