Re: Density calibration function

Posted by Wayne Rasband on Apr 05, 2006; 5:43pm
URL: http://imagej.273.s1.nabble.com/Density-calibration-function-tp3703200p3703201.html

I get pretty good fits when I paste this data set into the
Analyze>Tools>Curve Fitting window, delete the first point and last two
points, and then use either a 2nd degree polynomial or Rodbard
function. There is a screenshot at
<http://rsb.info.nih.gov/ij/images/curve-fits.gif>.

-wayne

> I am doing a project to move a program under NIH Image to
> ImageJ. However there is a problem on density calibration. I
> used same Rodbard function on ImageJ and NIH Image, but got
> the different result. It seems the NIH image gave better
> result. My calibration table is following:
>
> 17.08    .038
> 85.3    .607
> 96.9    .616
> 113.86    .657
> 119.66    .686
> 132.95    .733
> 151.06    .793
> 155.47    .821
> 169.65    .882
> 172.86    .896
> 186.42    .973
> 191.11    1.008
> 205.52    1.109
> 221.93    1.27
> 227.23    1.361
> 234.72    1.492
> 239.85    1.611
> 240.49    1.632
> 246.08    1.869
> 252.81    2.799
>
> Any thought? Thanks.