Graphing Nuclear Intensities

Posted by Robert Peterson-3-3 on Mar 23, 2006; 7:22pm
URL: http://imagej.273.s1.nabble.com/Find-bounding-box-coordinates-for-target-in-binary-mask-image-tp3703318p3703321.html

Hi,
I've been trying to do this on my own, but can't seem to combine all the
right elements.  What I need to do is the following:

   1. Pick the nuclei from a widefield fluorescence image visualized
      with an antibody that is very clean for only the nuclei (I have no
      problem doing this by itself).
   2. Determine the "intensity" of staining for each nuclei (this I'm
      having trouble figuring out).
   3. Graph the intensity versus number of nuclei to see the distribution.

I would appreciate any help.
Thanks,
Robert

--
Robert E. Peterson, Ph.D. - Assistant Professor
Confocal & Multiphoton Imaging Facility Director
UNC-Chapel Hill Neuroscience Center
105 Mason Farm Road, NRB Rm. 7109A
Campus Box 7250
Chapel Hill, NC 27599
[phone]