Re: Graphing Nuclear Intensities

Posted by Thomas Radtke on
URL: http://imagej.273.s1.nabble.com/Find-bounding-box-coordinates-for-target-in-binary-mask-image-tp3703318p3703322.html

Robert Peterson schrieb:

>   2. Determine the "intensity" of staining for each nuclei (this I'm
>      having trouble figuring out).

If you just need the intensity in arbitrary units, then you should be
fine with the sum of all grey values detected for each of your nuclei.
If you actually want to count the number of dyes, you would have to do
some sort of calibration. Of course, background subtraction is mandatory
for quantitative measurements in either case. Fading might be a problem
too.

Thomas