Posted by
Felipe J. Álvarez M. on
URL: http://imagej.273.s1.nabble.com/Image-transform-tp3703413p3703415.html
Dear Liu:
I don't know if this could be of help, but there is a plug-in called "radial profile" which maybe could help you.
fjam
"Liu, Dongfang (NIH/NIAID) [E]" <
[hidden email]> escribió:
Dear friends,
I am new user of Image J.
I am sorry to bother you.
I am looking for your help about the analysis of "radial intensity distribution" . The radial intensity distribution I(r) of the flourescence dot was fit for each frame with a nonlinear Levenberg-Marquardt routine to the Gaussian:I(r)=Io exp(-R^2/w^2) +BG, where R is the distance of each pixel to the center of mass. The fitting parameters are Io, w, and BG, where Io is the peak intensity, BG is the background intensity, and w is the measure of the width (the Gauss width). It is very important for us to get the peak intensity and width. some people suggest me to use Plot Profile(Analyze -> Plot.
But I think the "plot profile" is not good. Because the concept of "plot profile"---Displays a two-dimensional graph of the intensities of pixels along a line within the image. The x-axis represents distance along the line and the y-axis is the pixel intensity. For rectangular selections, displays a "column average plot", where the x-axis represents the horizontal distance through the selection and the y-axis the vertically averaged pixel intensity
I was confused that how to calculate the "radial intensity distribution" in Image Pro Plus or Image J or other software. Could you help me? Advice would be greatly appreciated.
I am looking forward to your reply.
Thank you very much.
Best regards
df
> ----------
> From: Gabriel Landini
> Reply To: List IMAGEJ
> Sent: Friday, March 3, 2006 12:10 PM
> To: List IMAGEJ
> Subject: Re: Image transform
>
> On Friday 03 March 2006 16:25, Liu, Dongfang (NIH/NIAID) [E] wrote:
> > Thank you for your help.
> > Yes, the system is a miroscope. Could you tell what other optics I should
> > consider to calibrate a image?
>
> No, I couldn't because I do not know what kind of optics your microscope has.
>
> You have to do this:
>
> > > You need to put a known
> > > sized object under your imaging system and see how many pixels it spans.
> > > Then you estimate the size of your pixels dividing the known size of your
> > > object by the number of pixels it occupies.
>
> Cheers,
>
> Gabriel
>
>
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