> ----------
> From: Felipe J. Álvarez M.
> Reply To: List IMAGEJ
> Sent: Monday, March 6, 2006 12:03 PM
> To: List IMAGEJ
> Subject: Re: Questions about analysis "radial intensity distribution"
>
> Dear Liu:
> I don't know if this could be of help, but there is a plug-in called "radial profile" which maybe could help you.
>
> fjam
>
> "Liu, Dongfang (NIH/NIAID) [E]" <
[hidden email]> escribió:
> Dear friends,
> I am new user of Image J.
> I am sorry to bother you.
> I am looking for your help about the analysis of "radial intensity distribution" . The radial intensity distribution I(r) of the flourescence dot was fit for each frame with a nonlinear Levenberg-Marquardt routine to the Gaussian:I(r)=Io exp(-R^2/w^2) +BG, where R is the distance of each pixel to the center of mass. The fitting parameters are Io, w, and BG, where Io is the peak intensity, BG is the background intensity, and w is the measure of the width (the Gauss width). It is very important for us to get the peak intensity and width. some people suggest me to use Plot Profile(Analyze -> Plot.
> But I think the "plot profile" is not good. Because the concept of "plot profile"---Displays a two-dimensional graph of the intensities of pixels along a line within the image. The x-axis represents distance along the line and the y-axis is the pixel intensity. For rectangular selections, displays a "column average plot", where the x-axis represents the horizontal distance through the selection and the y-axis the vertically averaged pixel intensity
> I was confused that how to calculate the "radial intensity distribution" in Image Pro Plus or Image J or other software. Could you help me? Advice would be greatly appreciated.
> I am looking forward to your reply.
>
> Thank you very much.
> Best regards
> df
>
>
> > ----------
> > From: Gabriel Landini
> > Reply To: List IMAGEJ
> > Sent: Friday, March 3, 2006 12:10 PM
> > To: List IMAGEJ
> > Subject: Re: Image transform
> >
> > On Friday 03 March 2006 16:25, Liu, Dongfang (NIH/NIAID) [E] wrote:
> > > Thank you for your help.
> > > Yes, the system is a miroscope. Could you tell what other optics I should
> > > consider to calibrate a image?
> >
> > No, I couldn't because I do not know what kind of optics your microscope has.
> >
> > You have to do this:
> >
> > > > You need to put a known
> > > > sized object under your imaging system and see how many pixels it spans.
> > > > Then you estimate the size of your pixels dividing the known size of your
> > > > object by the number of pixels it occupies.
> >
> > Cheers,
> >
> > Gabriel
> >
> >
>
>
>
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